Skip to main content

Shining a LAMP on the applications of isothermal amplification for monitoring environmental biosecurity

Bookreader Item Preview

texts

Shining a LAMP on the applications of isothermal amplification for monitoring environmental biosecurity

by
Deliveyne, Nathan; Young, Jennifer M.; Austin, Jeremy J.; Cassey, Phillip

Publication date
Usage
Attribution 4.0 InternationalCreative Commons Licenseby
Topics
Environmental biosecurity, invasive species, loop mediated isothermal amplification (LAMP), wildlife forensics
Publisher
Pensoft Publishers
Collection
biodiversity
Contributor
Pensoft Publishers
Language
English
Rights
https://biodiversitylibrary.org/permissions
Rights-holder
Copyright held by individual article author(s).
Volume
82
Item Size
30.8M
Abstract
Environmental biosecurity risks associated with the transnational wildlife trade include the loss of biodiversity, threats to public health, and the proliferation of invasive alien species. To assist enforcement agencies in identifying species either intentionally (trafficked) or unintentionally (stowaway) entrained in the trade-chain pathway, rapid forensic techniques are needed to enable their detection from DNA samples when physical identification is not possible. Loop Mediated Isothermal Amplification (LAMP) is an emerging technique, with recent applications in biosecurity and forensic sciences, which has potential to function as a field-based detection tool. Here we provide an overview of current research that applies LAMP to environmental biosecurity, including identification of ornamental wildlife parts, consumer products, and invasive species monitoring and biosecurity detection. We discuss the current scope of LAMP as applied to various wildlife trade scenarios and biosecurity checkpoint monitoring, highlight the specificity, sensitivity, and robustness for these applications, and review the potential utility of LAMP for rapid field-based detection at biosecurity checkpoints. Based on our assessment of the literature we recommend broader interest, research uptake, and investment in LAMP as an appropriate field-based species detection method for a wide range of environmental biosecurity scenarios.
Addeddate
2025-04-02 04:41:40
Bhl_virtual_titleid
210923
Bhl_virtual_volume
v.82 (2023)
Call number
10_3897_neobiota_82_97998
Call-number
10_3897_neobiota_82_97998
Foldoutcount
0
Genre
article
Identifier
shininglamponap82deli
Identifier-ark
ark:/13960/s2x14t1g9hd
Identifier-bib
10_3897_neobiota_82_97998
Identifier-doi
10.3897/neobiota.82.97998


comment
Reviews

149 Views

DOWNLOAD OPTIONS

download 1 file
B/W PDF download
download 1 file
CHOCR download
DAISY

For users with print-disabilities

EPUB
download 1 file
FULL TEXT download
download 1 file
HOCR download
download 1 file
ITEM TILE download
download 1 file
OCR PAGE INDEX download
download 1 file
OCR SEARCH TEXT download
download 1 file
PAGE NUMBERS JSON download
download 1 file
PDF download
download 1 file
SINGLE PAGE PROCESSED JP2 ZIP download
download 1 file
TORRENT download
download 17 Files
download 8 Original
SHOW ALL

IN COLLECTIONS

Biodiversity Heritage Library

Uploaded by Smithsonian Libraries and Archives on

SIMILAR ITEMS (based on metadata)

Terms of Service (last updated 12/31/2014)